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PDGFR beta Polyclonal antibody
PDGFR beta Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13449-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 31 publications below |
| IHC | See 6 publications below |
| IP | See 1 publications below |
Product Information
13449-1-AP targets PDGFR beta in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDGFR beta fusion protein Ag4285 |
| Full Name | platelet-derived growth factor receptor, beta polypeptide |
| Calculated Molecular Weight | 1106 aa, 124 kDa |
| Observed Molecular Weight | 160-180 kDa |
| GenBank Accession Number | BC032224 |
| Gene Symbol | PDGFR beta |
| Gene ID (NCBI) | 5159 |
| RRID | AB_2162644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGFR beta antibody 13449-1-AP | Download protocol |
| IHC protocol for PDGFR beta antibody 13449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.
| ||
Cell Rep Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells | ||
J Mol Cell Biol BMP4 mediates the interplay between adipogenesis and angiogenesis during expansion of subcutaneous white adipose tissue. | ||
Br J Cancer Inhibition of PFKFB3 in HER2-positive gastric cancer improves sensitivity to trastuzumab by inducing tumour vessel normalisation. | ||
Neurotherapeutics A switch in the pathway of TRPC3-mediated calcium influx into brain pericytes contributes to capillary spasms after subarachnoid hemorrhage |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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