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WB analysis of PC-3 using 16692-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 16692-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 16692-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 40x lens).

PARP11 Polyclonal antibody

Name: PARP11 Polyclonal antibody
Brand: Proteintech
SKU: 16692-1-AP

PARP11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16692-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

C12orf6, PARP 11, PARP11

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, PC-3 cells, mouse heart tissue, mouse spleen tissue
Positive IHC detected in	human kidney tissue, human placenta tissue, human colon tissue, human testis tissue, human skin tissue, human liver tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IF	See 1 publications below

Product Information

16692-1-AP targets PARP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PARP11 fusion protein Ag10053 Predict reactive species
Full Name	poly (ADP-ribose) polymerase family, member 11
Calculated Molecular Weight	331 aa, 39 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC017569
Gene Symbol	PARP11
Gene ID (NCBI)	57097
RRID	AB_2158504
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NR21
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP11, also named as ARTD11, belongs to the ARTD/PARP family. PARP11 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. PARP11 plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (PMID: 25043379). PARP11 has 4 isoforms with the molecular mass of 28-40 kDa.

Protocols

Product Specific Protocols
WB protocol for PARP11 antibody 16692-1-AP	Download protocol
IHC protocol for PARP11 antibody 16692-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,pig	WB,IF	Front Cell Infect Microbiol Identification of porcine PARP11 as a restricted factor for pseudorabies virus Authors - Chunyun Qi KD Validated View Article