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Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
27808-1-AP targets PARG in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARG fusion protein Ag27137 Predict reactive species |
| Full Name | poly (ADP-ribose) glycohydrolase |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 100-111 kDa |
| GenBank Accession Number | BC052966 |
| Gene Symbol | PARG |
| Gene ID (NCBI) | 8505 |
| RRID | AB_2880979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86W56 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PARG antibody 27808-1-AP | Download protocol |
| IHC protocol for PARG antibody 27808-1-AP | Download protocol |
| IP protocol for PARG antibody 27808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma. | ||
Int J Mol Sci Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum | ||
Onco Targets Ther Synergistic Effect of Ubiquitin-Specific Protease 14 and Poly(ADP-Ribose) Glycohydrolase Co-Inhibition in BRCA1-Mutant, Poly(ADP-Ribose) Polymerase Inhibitor-Resistant Triple-Negative Breast Cancer Cells | ||
Viruses Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus |
