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Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
14708-1-AP targets PAPSS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAPSS1 fusion protein Ag6401 Predict reactive species |
| Full Name | 3'-phosphoadenosine 5'-phosphosulfate synthase 1 |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC050627 |
| Gene Symbol | PAPSS1 |
| Gene ID (NCBI) | 9061 |
| RRID | AB_2159487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| IHC protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| IF protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| IP protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res CIB2 negatively regulates oncogenic signaling in ovarian cancer via sphingosine kinase 1. | ||
Biotechnol J Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. | ||
Histol Histopathol Correlation of 3'-Phosphoadenosine-5'-Phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous carcinoma | ||
bioRxiv Glycosaminoglycan-mediated lipoprotein uptake protects cancer cells from ferroptosis |
