Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11001-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11001-1-AP
IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug.
Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 11001-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
K-562 cells, human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human lung cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:10-1:100
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
11001-1-AP targets PANK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.
Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11001-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung cancer using 11001-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11001-1-AP (PANK2 antibody) at dilution of 1:10 (under 10x lens).
IHC staining of human liver using 11001-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11001-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse brain using 11001-1-AP
IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug.
IF/ICC Figures
IF Staining of HepG2 using 11001-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PANK2 Polyclonal antibody
Catalog Number
11001-1-AP
Citations
-
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:10-1:100 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
