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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells, mouse lung tissue |
| Positive IP detected in | A431 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
15583-1-AP targets PAIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAIP2 fusion protein Ag7119 Predict reactive species |
| Full Name | poly(A) binding protein interacting protein 2 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC001716 |
| Gene Symbol | PAIP2 |
| Gene ID (NCBI) | 51247 |
| RRID | AB_10950891 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BPZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAIP2 antibody 15583-1-AP | Download protocol |
| IF protocol for PAIP2 antibody 15583-1-AP | Download protocol |
| IP protocol for PAIP2 antibody 15583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity. | ||
Front Genet PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration | ||
Pharmacol Res Inhibition of K63 ubiquitination by G-Protein pathway suppressor 2 (GPS2) regulates mitochondria-associated translation |
