- Featured Product
- KD/KO Validated
PAF1 Polyclonal antibody
PAF1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15441-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
Product Information
15441-1-AP targets PAF1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAF1 fusion protein Ag7212 |
| Full Name | Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC000017 |
| Gene Symbol | PAF1 |
| Gene ID (NCBI) | 54623 |
| RRID | AB_2174457 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAF1, also known as hPAF1 or PD2, is a PAF (RNA Polymerase II Associated Factor) complex subunit. Paf1 functions primarily to modulates transcript elongation. PAF1 occupies transcriptional enhancers and restrains hyperactivation of a subset of these enhancers(PMID: 28860207). The PAF complex also has a role in histone monoubiquitination. Paf1 is overexpressed in pancreatic cancer cells and has demonstrated potential oncogenic property. The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAF1 antibody 15441-1-AP | Download protocol |
| IHC protocol for PAF1 antibody 15441-1-AP | Download protocol |
| IP protocol for PAF1 antibody 15441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.
| ||
J Cell Biol Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine. | ||
Radiat Oncol Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription. | ||
ACS Pharmacol Transl Sci Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening | ||
Sci Adv CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation | ||
