Hela lysates were subjected to SDS PAGE followed by western blot with 11960-1-AP (NPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Hela lysates were subjected to SDS PAGE followed by western blot with 11960-1-AP (NPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11960-1-AP
IP result of anti-NPM3 (IP:11960-1-AP, 4ug; Detection:11960-1-AP 1:500) with HeLa cells lysate 3400ug.
IP result of anti-NPM3 (IP:11960-1-AP, 4ug; Detection:11960-1-AP 1:500) with HeLa cells lysate 3400ug.
IHC staining of human lymphoma using 11960-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 11960-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11960-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11960-1-AP (NPM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11960-1-AP (NPM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
NPM3, a member of the nucleophosmin/ nucleoplasmin family, lacks intrinsic histone chaperone activity, inhibits histone assembly activity of NPM1 in vitro, and dramatically enhances transcription in a cellular system(PMID: 20073534). Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. The NPM3 protein migrated in SDS-PAGE gels as a doublet with apparent molecular masses of 25 and 27 kDa, which are larger than the 20.5 kDa mass predicted by the deduced amino acid sequence including the HA tag (PMID: 11722795). The NPM3 antibody can detect two bands with MW between 20-27 kDa.
Hela lysates were subjected to SDS PAGE followed by western blot with 11960-1-AP (NPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lymphoma using 11960-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 11960-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 11960-1-AP
IP result of anti-NPM3 (IP:11960-1-AP, 4ug; Detection:11960-1-AP 1:500) with HeLa cells lysate 3400ug.
IF/ICC Figures
IF Staining of HeLa using 11960-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11960-1-AP (NPM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
NPM3 Polyclonal antibody
Catalog Number
11960-1-AP
Citations
3
Dilutions
WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
