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  • Featured Product
  • KD/KO Validated

NOTCH3 Polyclonal antibody

NOTCH3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 55114-1-AP

Synonyms

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOTCH3 Polyclonal antibody (55114-1-AP)

    WB analysis using 55114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NOTCH3 Polyclonal antibody (55114-1-AP)

    WB analysis using 55114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NOTCH3 Polyclonal antibody (55114-1-AP)

    WB analysis of COLO 320 using 55114-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using NOTCH3 Polyclonal antibody (55114-1-AP)

    WB analysis of U2OS using 55114-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NOTCH3 Polyclonal antibody (55114-1-AP)

    IP experiment of HeLa using 55114-1-AP

    IP result of anti-NOTCH3 (IP:55114-1-AP, 4ug; Detection:55114-1-AP 1:800) with HeLa cells lysate 2000 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NOTCH3 Polyclonal antibody (55114-1-AP)

    IHC staining of human lung cancer using 55114-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NOTCH3 Polyclonal antibody (55114-1-AP)

    IHC staining of human lung cancer using 55114-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOTCH3 Polyclonal antibody (55114-1-AP)

    IHC staining of human breast cancer using 55114-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOTCH3 Polyclonal antibody (55114-1-AP)

    IHC staining of human breast cancer using 55114-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NOTCH3 Polyclonal antibody (55114-1-AP)

    IHC staining of human kidney using 55114-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NOTCH3 Polyclonal antibody (55114-1-AP)

    IHC staining of human kidney using 55114-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA2780 cells, Caco-2 cells, U2OS cells, COLO 320 cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55114-1-AP targets NOTCH3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Notch homolog 3 (Drosophila)
Calculated Molecular Weight 244 kDa
Observed Molecular Weight 250-260 kDa, 100 kDa
GenBank Accession NumberNM_000435
Gene Symbol NOTCH3
Gene ID (NCBI) 4854
RRIDAB_10858393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).

Protocols

Product Specific Protocols
WB protocol for NOTCH3 antibody 55114-1-APDownload protocol
IHC protocol for NOTCH3 antibody 55114-1-APDownload protocol
IP protocol for NOTCH3 antibody 55114-1-APDownload protocol
FC protocol for NOTCH3 antibody 55114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
View Article
human,mouse,ratWB,IF

Sci Transl Med

JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension.

Authors - Yu Zhang
View Article
mouseWB

Mol Ther

METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

Authors - Ling Jiang
View Article
ratWB

Biomaterials

Laminin-521 promotes quiescence in isolated stellate cells from rat liver.

Authors - Friederike Rohn
View Article
mouseWB

Cancer Res

Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction.

Authors - Shubing Zhang
View Article
humanWB

Oncogene

Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.

Authors - Sourik S Ganguly
  • KD Validated
View Article