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NFKB2 Monoclonal antibody

NFKB2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A10E9

Cat no : 66920-1-Ig

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFKB2 Monoclonal antibody (66920-1-Ig)

    WB analysis using 66920-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66920-1-Ig (NFKB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NFKB2 Monoclonal antibody (66920-1-Ig)

    IHC staining of human liver cancer using 66920-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NFKB2 Monoclonal antibody (66920-1-Ig)

    IHC staining of human pancreas cancer using 66920-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NFKB2 Monoclonal antibody (66920-1-Ig)

    IHC staining of human pancreas cancer using 66920-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NFKB2 Monoclonal antibody (66920-1-Ig)

    IHC staining of human liver cancer using 66920-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, K-562 cells, Daudi cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66920-1-Ig targets NFKB2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFKB2 fusion protein Ag28543
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC002844
Gene Symbol NFKB2
Gene ID (NCBI) 4791
RRIDAB_2882247
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Protocols

Product Specific Protocols
WB protocol for NFKB2 antibody 66920-1-IgDownload protocol
IHC protocol for NFKB2 antibody 66920-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols