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NFKB2 Polyclonal antibody

NFKB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 51085-1-AP

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2,p100, NFKB2,p52,p100, Oncogene Lyt 10, p100, p52

Validation Data Gallery

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  • Western Blot (WB) analysis of Apoptosised HeLa cells using NFKB2 Polyclonal antibody (51085-1-AP)

    WB analysis of Apoptosised HeLa using 51085-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 51085-1-AP (NFKB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inApoptosised HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51085-1-AP targets NFKB2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC002844
Gene Symbol NFKB2
Gene ID (NCBI) 4791
RRIDAB_2267124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NFKB2.

Protocols

Product Specific Protocols
WB protocol for NFKB2 antibody 51085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols