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  • Western Blot (WB) analysis of various lysates using NFATC3 Monoclonal antibody (66924-1-Ig)

    WB analysis using 66924-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thymus tissue using NFATC3 Monoclonal antibody (66924-1-Ig)

    IHC staining of human thymus using 66924-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thymus tissue using NFATC3 Monoclonal antibody (66924-1-Ig)

    IHC staining of human thymus using 66924-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NFATC3 Monoclonal antibody

NFATC3 Monoclonal Antibody for WB, ELISA
Cat No. 66924-1-Ig
Clone No.1C1H8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66924-1-Ig targets NFATC3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.16 x 10-10M
KOff=5.67 x 10-6M
KOn=4.90 x 104M
Immunogen NFATC3 fusion protein Ag27306 Predict reactive species
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC001050
Gene Symbol NFATC3
Gene ID (NCBI) 4775
RRIDAB_2882251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12968
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

Protocols

Product Specific Protocols
WB protocol for NFATC3 antibody 66924-1-IgDownload protocol
IHC protocol for NFATC3 antibody 66924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols