Filter: AllWBIPIHCIF/ICC

WB analysis using 21698-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 21698-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 21698-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21698-1-AP
WB result of NEDD4 antibody (21698-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEDD4 transfected HeLa cells.
WB analysis of HT-1080 using 21698-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 21698-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of C6 using 21698-1-AP
IP result of anti-NEDD4 (IP:21698-1-AP, 4ug; Detection:21698-1-AP 1:500) with C6 cells lysate 1600ug.
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human liver using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human liver using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 21698-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NEDD4 antibody (21698-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

NEDD4 Polyclonal antibody

Name: NEDD4 Polyclonal antibody
Brand: Proteintech
SKU: 21698-1-AP
Rating: 5.00 (1 reviews)

NEDD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21698-1-AP

Publications(53)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4, KIAA0093

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (53)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, C6 cells, HeLa cells, HT-1080 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, COS-7 cells
Positive IP detected in	C6 cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 49 publications below
IHC	See 5 publications below
IF	See 11 publications below
IP	See 7 publications below
CoIP	See 2 publications below

Product Information

21698-1-AP targets NEDD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NEDD4 fusion protein Ag16291 Predict reactive species
Full Name	neural precursor cell expressed, developmentally down-regulated 4
Calculated Molecular Weight	1319 aa, 149 kDa
Observed Molecular Weight	115 kDa
GenBank Accession Number	BC152452
Gene Symbol	NEDD4
Gene ID (NCBI)	4734
RRID	AB_10858626
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P46934
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2 (PMID: 23545411). NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain (PMID: 35328067). NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

Protocols

Product Specific Protocols
WB protocol for NEDD4 antibody 21698-1-AP	Download protocol
IHC protocol for NEDD4 antibody 21698-1-AP	Download protocol
IF protocol for NEDD4 antibody 21698-1-AP	Download protocol
IP protocol for NEDD4 antibody 21698-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Adv Sci (Weinh) OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model Authors - Xiaomeng Song View Article
mouse	WB	Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice Authors - Dingfeng Zou View Article
human	WB	J Exp Clin Cancer Res PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer Authors - Liang Xu View Article
mouse	WB	J Biomed Sci Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1 Authors - Yichen Wu View Article
mouse	WB	Proc Natl Acad Sci U S A Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis. Authors - Siming Li View Article
human	WB	Theranostics PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex. Authors - Xiaoxiao Wang View Article