Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 19977-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 19977-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse heart using 19977-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 19977-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 19977-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of C2C12 using 19977-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using MYBPC3 antibody (19977-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using MYBPC3 antibody (19977-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
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mouse heart tissue, rat heart tissue, human heart tissue
Positive IHC detected in
mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse heart tissue
Positive IF/ICC detected in
C2C12 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)
Case Report: Identification of the First Synonymous Variant of Myosin Binding Protein C3 (c.24A>C, p.P8P) Altering RNA Splicing in a Cardiomyopathy and Sudden Cardiac Death Case.
Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 19977-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 19977-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse heart using 19977-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 19977-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse heart using 19977-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 19977-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF/ICC Figures
IF Staining of C2C12 using 19977-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using MYBPC3 antibody (19977-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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