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  • Featured Product
  • KD/KO Validated

MTHFD1L Polyclonal antibody

MTHFD1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16113-1-AP

Synonyms

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTHFD1L Polyclonal antibody (16113-1-AP)

    WB analysis using 16113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using MTHFD1L Polyclonal antibody (16113-1-AP)

    WB analysis of mouse ovary using 16113-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using MTHFD1L Polyclonal antibody (16113-1-AP)

    WB analysis of COLO 320 using 16113-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MTHFD1L Polyclonal antibody (16113-1-AP)

    IP experiment of HeLa using 16113-1-AP

    IP result of anti-MTHFD1L (IP:16113-1-AP, 4ug; Detection:16113-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1L Polyclonal antibody (16113-1-AP)

    IHC staining of human liver cancer using 16113-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1L Polyclonal antibody (16113-1-AP)

    IHC staining of human liver cancer using 16113-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1L Polyclonal antibody (16113-1-AP)

    IHC staining of human liver cancer using 16113-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFD1L Polyclonal antibody (16113-1-AP)

    IHC staining of human liver cancer using 16113-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTHFD1L Polyclonal antibody (16113-1-AP)

    IF Staining of HepG2 using 16113-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16113-1-AP (MTHFD1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, COLO 320 cells, mouse ovary tissue, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16113-1-AP targets MTHFD1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTHFD1L fusion protein Ag9036
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Calculated Molecular Weight 978 aa, 106 kDa
Observed Molecular Weight 106 kDa
GenBank Accession NumberBC017477
Gene Symbol MTHFD1L
Gene ID (NCBI) 25902
RRIDAB_2250974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MTHFD1L antibody 16113-1-APDownload protocol
IHC protocol for MTHFD1L antibody 16113-1-APDownload protocol
IF protocol for MTHFD1L antibody 16113-1-APDownload protocol
IP protocol for MTHFD1L antibody 16113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.

Authors - Saara Forsström
View Article
humanWB

Cell Metab

Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.

Authors - Won Dong Lee
View Article
humanWB

Nat Metab

Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells

Authors - Alanna C Green
View Article
human, mouseWB

Cell Metab

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Authors - Joni Nikkanen
View Article
humanWB, IHC

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui
  • KD Validated
View Article
humanWB

Nat Commun

Increased formate overflow is a hallmark of oxidative cancer.

Authors - Johannes Meiser
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Furkan Emre (Verified Customer) (02-21-2022)

I am satisfied with the antibody. It worked as I was expecting from it. I would buy it again. I also add a figure showing chemiluminescence detection of MTHFD1L using your antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human ES2 cells
MTHFD1L Antibody Western Blot validation (1:1000 dilution) in Human ES2 cells (Cat no:16113-1-AP)