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MPP8 Polyclonal antibody
MPP8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 16796-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 19 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 4 publications below |
| CoIP | See 2 publications below |
| ChIP | See 3 publications below |
Product Information
16796-1-AP targets MPP8 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MPP8 fusion protein Ag10268 |
| Full Name | M-phase phosphoprotein 8 |
| Calculated Molecular Weight | 860 aa, 97 kDa |
| Observed Molecular Weight | 97-105 kDa |
| GenBank Accession Number | BC003542 |
| Gene Symbol | MPP8 |
| Gene ID (NCBI) | 54737 |
| RRID | AB_2266644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a) [PMID: 18726070].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPP8 antibody 16796-1-AP | Download protocol |
| IF protocol for MPP8 antibody 16796-1-AP | Download protocol |
| IP protocol for MPP8 antibody 16796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
| ||
Nat Genet Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2. | ||
Nat Genet Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia. | ||
Mol Cell Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression. | ||
Nat Microbiol Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Roy (Verified Customer) (06-12-2024) | Great antibody to IP MPP8, working well on WB as well (1/1000, 1h at RT) in HeLa cells and Jurkat cells.
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