mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 51142-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 51142-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 51142-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 51142-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunohistochemistry (IHC)
IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
51142-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 51142-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 51142-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 51142-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 51142-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
MAP2K4 Polyclonal antibody
$149 (20ul) $449 (150ul)
Catalog Number
51142-1-AP
Citations
-
Dilutions
WB : 1:500-1:1000 IHC : 1:20-1:200
Applications
WB, IHC, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
