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  • Featured Product
  • KD/KO Validated

LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 19987-1-AP

Synonyms

LDHA, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis of HeLa using 19987-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis of Jurkat using 19987-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis of Jurkat using 19987-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis of mouse testis using 19987-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis of A431 using 19987-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    WB analysis of rat kidney using 19987-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IP experiment of mouse kidney using 19987-1-AP

    IP result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human heart using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human heart using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human testis using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human testis using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IF Staining of HepG2 using 19987-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LDHA-Specific Polyclonal antibody (19987-1-AP)

    IF Staining of HepG2 using 19987-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19987-1-AP targets LDHA-Specific in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, chicken, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lactate dehydrogenase A
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberNM_005566
Gene Symbol LDHA
Gene ID (NCBI) 3939
RRIDAB_10646429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

Protocols

Product Specific Protocols
WB protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IHC protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IF protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IP protocol for LDHA-Specific antibody 19987-1-APDownload protocol
FC protocol for LDHA-Specific antibody 19987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
View Article
mouseWB

Nat Cell Biol

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Authors -
View Article
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
View Article
humanWB

Drug Resist Updat

Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression

Authors - Shuai Wang
  • KD Validated
View Article
human

Cell Stem Cell

Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury

Authors - Shuyong Wang
View Article
human

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Christian (Verified Customer) (01-07-2022)

We expected a huge expression in our primary human chondrocytes and it was perfectly and specifically confirmed by this antibody. The antibody worked also for primary rat chondrocytes. (cell lysis with RIPA-lysis buffer, denaturating conditions)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human primary chondrocytes
LDHA-Specific Antibody Western Blot validation (1:2000 dilution) in human primary chondrocytes (Cat no:19987-1-AP)