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  • Featured Product
  • KD/KO Validated

NGAL Polyclonal antibody

NGAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26991-1-AP

Synonyms

lipocalin 2, LCN2, Lcn 2, HNL, 25 kDa alpha-2-microglobulin-related subunit of MMP-9

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using NGAL Polyclonal antibody (26991-1-AP)

    WB analysis of human saliva using 26991-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NGAL Polyclonal antibody (26991-1-AP)

    WB analysis using 26991-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using NGAL Polyclonal antibody (26991-1-AP)

    WB analysis of HT-29 using 26991-1-AP

    WB result of NGAL antibody (26991-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human colon cancer using 26991-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human colon cancer using 26991-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human stomach cancer using 26991-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human stomach cancer using 26991-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NGAL Polyclonal antibody (26991-1-AP)

    IF Staining of A431 using 26991-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Lipocalin-2/NGAL antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using NGAL Polyclonal antibody (26991-1-AP)

    FC experiment of A431 using 26991-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Lipocalin-2/NGAL (26991-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHT-29 cells, human saliva
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26991-1-AP targets NGAL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGAL fusion protein Ag25715
Full Name lipocalin 2
Observed Molecular Weight 25 kDa
Gene Symbol NGAL/LCN2
Gene ID (NCBI) 3934
RRIDAB_2880715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

Protocols

Product Specific Protocols
WB protocol for NGAL antibody 26991-1-APDownload protocol
IHC protocol for NGAL antibody 26991-1-APDownload protocol
IF protocol for NGAL antibody 26991-1-APDownload protocol
FC protocol for NGAL antibody 26991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neurol Neurosurg Psychiatry

Molecules of senescent glial cells differentiate Alzheimer's disease from ageing

Authors - Linbin Dai
View Article
WB

Cell Chem Biol

3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.

Authors - Chenwen Shao
View Article
humanWB

Oxid Med Cell Longev

Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation.

Authors - Su Zhao
  • KD Validated
View Article
humanWB,IHC

Gastric Cancer

Lipocalin-2 negatively regulates epithelial-mesenchymal transition through matrix metalloprotease-2 downregulation in gastric cancer.

Authors - Sadaaki Nishimura
  • KD Validated
View Article
humanWB,IHC

Biochim Biophys Acta Mol Basis Dis

Lipocalin-2-mediated intestinal epithelial cells pyroptosis via NF-κB/NLRP3/GSDMD signaling axis adversely affects inflammation in colitis

Authors - Yuyi Yang
View Article
humanIF

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
View Article