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Tested Applications
| Positive WB detected in | HepG2 cells, human kidney tissue, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
13156-1-AP targets KLF12 in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLF12 fusion protein Ag3788 Predict reactive species |
| Full Name | Kruppel-like factor 12 |
| Calculated Molecular Weight | 402 aa, 44 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC019680 |
| Gene Symbol | KLF12 |
| Gene ID (NCBI) | 11278 |
| RRID | AB_2132260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4X4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KLF12, also named as AP2REP or HSPC122, is a 402 amino acid protein, which contains three C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. KLF12 localizes in the nucleus and confers strong transcriptional repression to the AP-2-alpha gene. KLF12 is atranscriptional silencer to the AP2-alpha gene, mediating positive and negative regulation of the AP2-A expression trhough a set of overlapping cis-regulatory promoters elements.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF12 antibody 13156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol circ_NOTCH3 Functions as a Protooncogene Competing With miR-205-5p, Modulating KLF12 Expression and Promoting the Development and Progression of Basal-Like Breast Carcinoma. | ||
J Ethnopharmacol Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose. | ||
Onco Targets Ther The role of miR-382-5p in glioma cell proliferation, migration and invasion. | ||
Biochem Biophys Res Commun Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization. | ||
Genes Dis All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members. |
