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  • KD/KO Validated

KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 13105-1-AP

Synonyms

Kinesin-2, Kinesin like protein KIF2A, kinesin heavy chain member 2A, Kinesin 2, KIF2



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, MDCK cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13105-1-AP targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF2A fusion protein Ag3748
Full Name kinesin heavy chain member 2A
Calculated Molecular Weight 679 aa, 77 kDa
Observed Molecular Weight 80-100 kDa
GenBank Accession NumberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KIF2A antibody 13105-1-APDownload protocol
IHC protocol for KIF2A antibody 13105-1-APDownload protocol
IF protocol for KIF2A antibody 13105-1-APDownload protocol
IP protocol for KIF2A antibody 13105-1-APDownload protocol
FC protocol for KIF2A antibody 13105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network

Authors - Cuirong Guan
  • KD Validated
humanWB, IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
mouseWB,IF

EMBO Rep

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

Authors - Jia-Qian Ju
humanWB

Front Oncol

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Authors - Xigang Xia
humanWB

Biomed Pharmacother

KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.

Authors - Xin Zhang
  • KD Validated
humanIF

Mol Biol Cell

Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.

Authors - Hongdan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Dorota (Verified Customer) (04-10-2019)

Good for western blotting.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250 -1:1000
  • Cell Tissue Type: U2OS
KIF2A Antibody Western Blot, validation (1:250 -1:1000 dilution) in U2OS (Cat no:13105-1-AP)