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  • Western Blot (WB) analysis of various lysates using KIAA1217 Polyclonal antibody (24880-1-AP)

    WB analysis using 24880-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using KIAA1217 Polyclonal antibody (24880-1-AP)

    WB analysis of mouse colon using 24880-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KIAA1217 Polyclonal antibody (24880-1-AP)

    WB analysis using 24880-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KIAA1217 Polyclonal antibody (24880-1-AP)

    WB analysis using 24880-1-AP

    Rat colon lysates were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

KIAA1217 Polyclonal antibody

KIAA1217 Polyclonal Antibody for WB, ELISA
Cat No. 24880-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA1217, Sickle tail protein homolog, SKT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 800 ug/ml
20 μL, 800 ug/ml
150 μL, 800 ug/ml

$449/ 150 μL

In stock in Rosemont, IL. Order within 12 hours and 51 mins to receive by Thursday, April 10

Freight/Packing: $40.00

Quantity

- +

Request bulk or custom quote

Buy two full size antibodies and get a third full size primary antibody for free. Use promo code B2G125.

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Tested Applications

Positive WB detected inHEK-293 cells, rat colon tissue, mouse colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24880-1-AP targets KIAA1217 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1217 fusion protein Ag19367 Predict reactive species
Full Name KIAA1217
Calculated Molecular Weight 1943 aa, 214 kDa
Observed Molecular Weight100-110 kDa, 140-160 kDa, 210 kDa
GenBank Accession NumberBC018764
Gene Symbol KIAA1217
Gene ID (NCBI) 56243
RRIDAB_2879775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5P2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.

Protocols

Product Specific Protocols
WB protocol for KIAA1217 antibody 24880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols