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KHDC1 Polyclonal antibody

KHDC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23443-1-AP

Synonyms

NDG1, KHDC 1, KH homology domain-containing protein 1, C6orf148, C6orf147

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using KHDC1 Polyclonal antibody (23443-1-AP)

    WB analysis of L02 using 23443-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 23443-1-AP (KHDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KHDC1 Polyclonal antibody (23443-1-AP)

    IHC staining of human stomach cancer using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KHDC1 Polyclonal antibody (23443-1-AP)

    IHC staining of human stomach cancer using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using KHDC1 Polyclonal antibody (23443-1-AP)

    IHC staining of human liver using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using KHDC1 Polyclonal antibody (23443-1-AP)

    IHC staining of human liver using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using KHDC1 Polyclonal antibody (23443-1-AP)

    IHC staining of human stomach using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using KHDC1 Polyclonal antibody (23443-1-AP)

    IHC staining of human stomach using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using KHDC1 Polyclonal antibody (23443-1-AP)

    IHC staining of human stomach using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using KHDC1 Polyclonal antibody (23443-1-AP)

    IF Staining of L02 using 23443-1-AP

    Immunofluorescent analysis of L02 cells using 23443-1-AP (KHDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman stomach cancer tissue, human liver tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23443-1-AP targets KHDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KHDC1 fusion protein Ag20115
Full Name KH homology domain containing 1
Calculated Molecular Weight 237 aa, 27 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC022080
Gene Symbol KHDC1
Gene ID (NCBI) 80759
RRIDAB_2879280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KHDC1, also named as C6orf148, belongs to the KHDC1 family. KHDC1 has two isoforms with calculated MW 27 kDa and 18 kDa. It's a membrane protein. We got 35-40 kDa in our WB detection.

Protocols

Product Specific Protocols
WB protocol for KHDC1 antibody 23443-1-APDownload protocol
IHC protocol for KHDC1 antibody 23443-1-APDownload protocol
IF protocol for KHDC1 antibody 23443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols