JNK Polyclonal antibody

Name: JNK Polyclonal antibody
Brand: Proteintech
SKU: 24164-1-AP

JNK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

161

Review Product

Cat no : 24164-1-AP

Synonyms

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 24164-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 24164-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 24164-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 24164-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24164-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24164-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 24164-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 24164-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24164-1-AP (JNK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 24164-1-AP
IP result of anti-JNK (IP:24164-1-AP, 4ug; Detection:24164-1-AP 1:10000) with A431 cells lysate 1280 ug.
IHC staining of human colon cancer using 24164-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 24164-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 24164-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 24164-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24164-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Jurkat using 24164-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (24164-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 24164-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (24164-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 24164-1-AP

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Tested Applications

Positive WB detected in	A431 cells, Jurkat cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human colon cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Jurkat cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 157 publications below
IHC	See 5 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

24164-1-AP targets JNK in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	JNK fusion protein Ag21426
Full Name	mitogen-activated protein kinase 8
Calculated Molecular Weight	48 kDa
Observed Molecular Weight	42 kDa, 50 kDa
GenBank Accession Number	BC130572
Gene Symbol	JNK
Gene ID (NCBI)	5599
RRID	AB_2879443
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 24164-1-AP	Download protocol
IHC protocol for JNK antibody 24164-1-AP	Download protocol
IF protocol for JNK antibody 24164-1-AP	Download protocol
IP protocol for JNK antibody 24164-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Mol Cell PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis Authors - Shouqiao Hou View Article
human	WB	Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression Authors - Xingran Li View Article
mouse	WB	Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response Authors - Zhe Wang View Article
mouse	WB	Transl Res Mitochondria transplantation protects traumatic brain injury via promoting neuronal survival and astrocytic BDNF. Authors - Jiqian Zhao View Article
human	WB	J Immunother Cancer TSH-TSHR axis promotes tumor immune evasion. Authors - Zhenghao Wu View Article
human	WB	Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A Authors - Lu Zhang View Article

JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 24164-1-AP

WB analysis using 24164-1-AP

WB analysis using 24164-1-AP

WB analysis of NIH/3T3 using 24164-1-AP

WB analysis of HEK-293 using 24164-1-AP

WB analysis of HeLa using 24164-1-AP

WB analysis using 24164-1-AP

WB analysis using 24164-1-AP

IP experiment of A431 using 24164-1-AP

IHC staining of human colon cancer using 24164-1-AP

IHC staining of human colon cancer using 24164-1-AP

IHC staining of human colon cancer using 24164-1-AP

IHC staining of human stomach cancer using 24164-1-AP

IF Staining of Jurkat using 24164-1-AP

FC experiment of HeLa using 24164-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Function

Tissue specificity

Involvement in disease

Isoforms

Post-translational modifications

Cellular localization

Protocols

Publications

PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis

OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

Mitochondria transplantation protects traumatic brain injury via promoting neuronal survival and astrocytic BDNF.

TSH-TSHR axis promotes tumor immune evasion.

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A