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  • Featured Product
  • KD/KO Validated

Integrin alpha 6 Polyclonal antibody

Integrin alpha 6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27189-1-AP

Synonyms

ITGA6, integrin, alpha 6, Integrin alpha-6 light chain, Integrin alpha-6 heavy chain, Integrin alpha-6

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Integrin Alpha 6 Polyclonal antibody (27189-1-AP)

    WB analysis of A549 using 27189-1-AP

    WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using Integrin Alpha 6 Polyclonal antibody (27189-1-AP)

    WB analysis using 27189-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Integrin Alpha 6 Polyclonal antibody (27189-1-AP)

    WB analysis of HepG2 using 27189-1-AP

    WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.

  • Western Blot (WB) analysis of A431 cells using Integrin Alpha 6 Polyclonal antibody (27189-1-AP)

    WB analysis of A431 using 27189-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using Integrin Alpha 6 Polyclonal antibody (27189-1-AP)

    WB analysis of mouse skin using 27189-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using Integrin Alpha 6 Polyclonal antibody (27189-1-AP)

    WB analysis of rat lung using 27189-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Integrin alpha 6 Polyclonal antibody (27189-1-AP)

    IHC staining of mouse skin using 27189-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Integrin alpha 6 Polyclonal antibody (27189-1-AP)

    IHC staining of mouse skin using 27189-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Integrin alpha 6 Polyclonal antibody (27189-1-AP)

    IHC staining of human colon using 27189-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Integrin alpha 6 Polyclonal antibody (27189-1-AP)

    IHC staining of human colon using 27189-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, A431 cells, mouse skin tissue, rat lung tissue, HepG2 cells
Positive IHC detected inmouse skin tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27189-1-AP targets Integrin alpha 6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, bovine, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin alpha 6 fusion protein Ag25835
Full Name integrin, alpha 6
Calculated Molecular Weight 1073 aa, 119 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC136456
Gene Symbol Integrin alpha 6
Gene ID (NCBI) 3655
RRIDAB_2880792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 6 antibody 27189-1-APDownload protocol
IHC protocol for Integrin alpha 6 antibody 27189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Appl Mater Interfaces

A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells

Authors - Xinyang Zhao
View Article
bovineIF

Cells

Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak

Authors - Xiaolan Zhang
View Article
monkeyWB

Virulence

Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.

Authors - Yuchen Li
View Article
humanWB

Stem Cell Res Ther

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Authors - Ling Leng
View Article
HamsterIF

J Virol

Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.

Authors - Xiu Xin
View Article
ratIF

Vascul Pharmacol

Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6

Authors - Li Wu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Insa (Verified Customer) (03-22-2023)

Did work very well in ICC

  • Applications: Other
  • Primary Antibody Dilution: 1:100-1:400
  • Cell Tissue Type: iPSC derived Astrocytes