Rabbit IgG control Polyclonal antibody
Rabbit IgG control Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
n/a
Applications
WB, IHC, IP, CoIP, ChIP, RIP, ELISA, Blocking assay, Cell treatment
Conjugate
Unconjugated
387
Cat no : 30000-0-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Control |
| Positive IP detected in | Control |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30000-0-AP targets Rabbit IgG control in WB, IHC, IP, CoIP, ChIP, RIP, ELISA, Blocking assay, Cell treatment applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N/A |
| Full Name | Rabbit IgG control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2819035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Publications
| Species | Application | Title |
|---|---|---|
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis | ||
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling | ||
Nat Commun m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation | ||
Nat Commun ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Siddharth (Verified Customer) (08-02-2023) | Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.
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FH Macarena (Verified Customer) (10-07-2022) | very concentrated. Excellent results. Deffinetly use less than you would normally use.
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FH Guo-rong (Verified Customer) (08-30-2022) | It works well as an antibody control in neutralization experiments
|
FH Anita (Verified Customer) (01-04-2022) | Works well as a control for IP
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FH Kenneth (Verified Customer) (02-14-2019) | Great company with Great products. Highly recommended.
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FH Priyanka (Verified Customer) (12-18-2018) |
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FH Seongjin (Verified Customer) (12-14-2018) |
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