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IVD Monoclonal antibody

IVD Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2D12A5

Cat no : 66032-1-Ig

Synonyms

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis using 66032-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis of MCF-7 using 66032-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis of human liver using 66032-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IVD Monoclonal antibody (66032-1-Ig)

    WB analysis of HEK-293 using 66032-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver cancer using 66032-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver using 66032-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using IVD Monoclonal antibody (66032-1-Ig)

    IHC staining of human liver using 66032-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IF Staining of human liver cancer using 66032-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using IVD Monoclonal antibody (66032-1-Ig)

    IF Staining of human liver cancer using 66032-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using IVD Monoclonal antibody (66032-1-Ig)

    IF Staining of MCF-7 using 66032-1-Ig

    Immunofluorescent analysis of MCF-7 cells, using IVD antibody 66032-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHEK-293 cells, human liver tissue, MCF-7 cells, Jurkat cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66032-1-Ig targets IVD in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IVD fusion protein Ag18042
Full Name isovaleryl Coenzyme A dehydrogenase
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 41-46 kDa
GenBank Accession NumberBC017202
Gene Symbol IVD
Gene ID (NCBI) 3712
RRIDAB_11042768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for IVD antibody 66032-1-IgDownload protocol
IHC protocol for IVD antibody 66032-1-IgDownload protocol
IF protocol for IVD antibody 66032-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Arch Toxicol

Activation of peroxisome proliferator-activated receptor α ameliorates perfluorododecanoic acid-induced production of reactive oxygen species in rat liver.

Authors - Hui Liu
View Article