- Featured Product
- KD/KO Validated
IFITM1 Polyclonal antibody
IFITM1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (2)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11727-3-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, K-562 cells |
Positive IP detected in | ROS1728 cells |
Positive IHC detected in | human lymphoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 21 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
FC | See 4 publications below |
Product Information
11727-3-AP targets IFITM1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, goat, African green monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFITM1 fusion protein Ag2320 |
Full Name | interferon induced transmembrane protein 1 (9-27) |
Calculated Molecular Weight | 14 kDa |
Observed Molecular Weight | 14-17 kDa |
GenBank Accession Number | BC000897 |
Gene Symbol | IFITM1 |
Gene ID (NCBI) | 8519 |
RRID | AB_2122083 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IFITM1 antibody 11727-3-AP | Download protocol |
IHC protocol for IFITM1 antibody 11727-3-AP | Download protocol |
IP protocol for IFITM1 antibody 11727-3-AP | Download protocol |
FC protocol for IFITM1 antibody 11727-3-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Mitotic progression following DNA damage enables pattern recognition within micronuclei. | ||
Nature Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.
| ||
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
Mol Ther Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus. | ||
Nat Commun IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses | ||
Cell Rep IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.
|