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  • Featured Product
  • KD/KO Validated

HSP27 Polyclonal antibody

HSP27 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18284-1-AP

Synonyms

HSPB1, HSP28, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP27 Polyclonal antibody (18284-1-AP)

    WB analysis using 18284-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HSP27 Polyclonal antibody (18284-1-AP)

    WB analysis using 18284-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HSP27 Polyclonal antibody (18284-1-AP)

    WB analysis of HeLa using 18284-1-AP

    WB result of HSP27 antibody (18284-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse liver tissue using HSP27 Polyclonal antibody (18284-1-AP)

    WB analysis of mouse liver using 18284-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using HSP27 Polyclonal antibody (18284-1-AP)

    WB analysis of C6 using 18284-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human lung cancer using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human lung cancer using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human liver cancer using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human liver cancer using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human gliomas using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human gliomas using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human lung cancer using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSP27 Polyclonal antibody (18284-1-AP)

    IHC staining of human lung cancer using 18284-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP27 Polyclonal antibody (18284-1-AP)

    IF Staining of HeLa using 18284-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP27 antibody (18284-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP27 Polyclonal antibody (18284-1-AP)

    IF Staining of HeLa using 18284-1-AP

    Immunofluorescent analysis of Hela cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HSP27 Polyclonal antibody (18284-1-AP)

    IF Staining of MCF-7 using 18284-1-AP

    Immunofluorescent analysis of MCF-7 cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using HSP27 Polyclonal antibody (18284-1-AP)

    FC experiment of MCF-7 using 18284-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (18284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, C6 cells, mouse liver tissue, HepG2 cells, U2OS cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18284-1-AP targets HSP27 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP27 fusion protein Ag13161
Full Name heat shock 27kDa protein 1
Calculated Molecular Weight 19-23 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC012768
Gene Symbol HSPB1
Gene ID (NCBI) 3315
RRIDAB_2295540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.

Protocols

Product Specific Protocols
WB protocol for HSP27 antibody 18284-1-APDownload protocol
IHC protocol for HSP27 antibody 18284-1-APDownload protocol
IF protocol for HSP27 antibody 18284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Oxidative cyclization reagents reveal tryptophan cation-π interactions

Authors - Xiao Xie
View Article
humanIF

Allergy

ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor?

Authors - Concepció Marin
View Article

Autophagy

ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.

Authors - Cabrera Sandra S
View Article
ratIF

J Cell Biol

Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.

Authors - Sebastian Kötter
View Article
humanWB,CoIP

Cell Rep

Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis

Authors - Konstantinos Tsioras
View Article
humanWB

Phytomedicine

Ginsenoside Rg5 inhibits glioblastoma by activating ferroptosis via NR3C1/HSPB1/NCOA4

Authors - Guoqing Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
HSP27 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:18284-1-AP)