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HSP27 Polyclonal antibody

HSP27 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse and More (2)





Cat no : 18284-1-AP


Heat shock protein beta 1, HSP 27, Hsp25, HSP27, HSP28, HSPB1

Tested Applications

Positive WB detected inA549 cells, C6 cells, mouse liver tissue, HeLa cells, HepG2 cells, U2OS cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18284-1-AP targets HSP27 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, fish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP27 fusion protein Ag13161
Full Name heat shock 27kDa protein 1
Calculated molecular weight 19-23 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC012768
Gene symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.


Product Specific Protocols
WB protocol for HSP27 antibody 18284-1-APDownload protocol
IHC protocol for HSP27 antibody 18284-1-APDownload protocol
IF protocol for HSP27 antibody 18284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor?

Authors - Concepció Marin


ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.

Authors - Cabrera Sandra S

J Cell Biol

Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.

Authors - Sebastian Kötter

Antioxid Redox Signal

Peroxiredoxin 6 is a crucial factor in the initial step of mitochondrial clearance and is upstream of the PINK1-Parkin pathway.

Authors - Shuaipeng Ma

CNS Neurosci Ther

FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling

Authors - Fan Zhang

Food Res Int

Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).

Authors - Lihong Ge


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
HSP27 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:18284-1-AP)