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  • Featured Product
  • KD/KO Validated

GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14700-1-AP

Synonyms

Cell and organelle markers, ECGP, endoplasmic reticulum marker, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP94 Polyclonal antibody (14700-1-AP)

    WB analysis using 14700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14700-1-AP (GRP94 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GRP94 Polyclonal antibody (14700-1-AP)

    WB analysis of HEK-293 using 14700-1-AP

    HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 14700-1-AP (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GRP94 Polyclonal antibody (14700-1-AP)

    IP experiment of mouse liver using 14700-1-AP

    IP Result of anti-GRP94 (IP:14700-1-AP, 3ug; Detection:14700-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GRP94 Polyclonal antibody (14700-1-AP)

    IP experiment of mouse liver using 14700-1-AP

    IP result of anti-GRP94(IP:14700-1-AP, 4ug; Detection:14700-1-AP 1:2000) with mouse liver tissue lysate 1560 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP94 Polyclonal antibody (14700-1-AP)

    IHC staining of human breast cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP94 Polyclonal antibody (14700-1-AP)

    IHC staining of human breast cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GRP94 Polyclonal antibody (14700-1-AP)

    IHC staining of human cervical cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 14700-1-AP (HSP90B1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GRP94 Polyclonal antibody (14700-1-AP)

    IHC staining of human cervical cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 14700-1-AP (HSP90B1 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRP94 Polyclonal antibody (14700-1-AP)

    IF Staining of HepG2 using 14700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP94 antibody (14700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GRP94 Polyclonal antibody (14700-1-AP)

    IF Staining of A431 using 14700-1-AP

    Immunofluorescent analysis of A431 cells, using HSP90B1 antibody 14700-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GRP94 Polyclonal antibody (14700-1-AP)

    IF Staining of HeLa using 14700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRP94 antibody (14700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRP94 Polyclonal antibody (14700-1-AP)

    IF Staining of HepG2 using 14700-1-AP

    Immunofluorescent analysis of HepG2 cells, using HSP90B1 antibody 14700-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HeLa cells using GRP94 Polyclonal antibody (14700-1-AP)

    FC experiment of HeLa using 14700-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (14700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HEK-293T cells using GRP94 Polyclonal antibody (14700-1-AP)

    FC experiment of HEK-293T using 14700-1-AP

    1X10^6 HEK-293T cells were stained with .2ug GRP94 antibody (14700-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells, A431 cells
Positive FC detected inHeLa cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

14700-1-AP targets GRP94 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP94 fusion protein Ag6344
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 92 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC066656
Gene symbol HSP90B1
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the C-terminal region of full-length HSP90b1.

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 14700-1-APDownload protocol
IHC protocol for GRP94 antibody 14700-1-APDownload protocol
IF protocol for GRP94 antibody 14700-1-APDownload protocol
IP protocol for GRP94 antibody 14700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
View Article
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
View Article
humanWB,IF

Theranostics

Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.

Authors - Zhiwei Liao
View Article
ratWB

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang
View Article
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
View Article
mouseWB

Antioxid Redox Signal

PMID: 27565538

Authors - Tianhe Li
View Article