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GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14700-1-AP

Synonyms

Cell and organelle markers, ECGP, endoplasmic reticulum marker, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14700-1-AP targets GRP94 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP94 fusion protein Ag6344
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC066656
Gene Symbol GRP94
Gene ID (NCBI) 7184
RRIDAB_2233347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 14700-1-APDownload protocol
IHC protocol for GRP94 antibody 14700-1-APDownload protocol
IF protocol for GRP94 antibody 14700-1-APDownload protocol
IP protocol for GRP94 antibody 14700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
humanWB,IF

Theranostics

Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.

Authors - Zhiwei Liao
ratWB

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
mouseWB

Antioxid Redox Signal

PMID: 27565538

Authors - Tianhe Li