Validation Data Gallery
|Positive WB detected in||A431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human breast cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells, A431 cells|
|Positive FC detected in||HeLa cells, HEK-293T cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
14700-1-AP targets GRP94 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GRP94 fusion protein Ag6344|
|Full Name||heat shock protein 90kDa beta (Grp94), member 1|
|Calculated molecular weight||92 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC066656|
|Gene ID (NCBI)||7184|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the C-terminal region of full-length HSP90b1.
|Product Specific Protocols|
|WB protocol for GRP94 antibody 14700-1-AP||Download protocol|
|IHC protocol for GRP94 antibody 14700-1-AP||Download protocol|
|IF protocol for GRP94 antibody 14700-1-AP||Download protocol|
|IP protocol for GRP94 antibody 14700-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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