- Featured Product
- KD/KO Validated
HSP90AB1 Polyclonal antibody
HSP90AB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 11405-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue, rat thymus tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 27 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 3 publications below |
| CoIP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
11405-1-AP targets HSP90AB1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, sheep, deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSP90AB1 fusion protein Ag1947 |
| Full Name | heat shock protein 90kDa alpha (cytosolic), class B member 1 |
| Calculated Molecular Weight | 723 aa, 83 kDa |
| Observed Molecular Weight | 83-90 kDa |
| GenBank Accession Number | BC014485 |
| Gene Symbol | HSP90AB1 |
| Gene ID (NCBI) | 3326 |
| RRID | AB_2121207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
| IHC protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
| IF protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
| IP protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
| FC protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. | ||
Haematologica Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. | ||
Anal Chem Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale. | ||
PLoS Pathog A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion. | ||
Int J Mol Sci A Comparative Transcriptomic Analysis Reveals That HSP90AB1 Is Involved in the Immune and Inflammatory Responses to Porcine Deltacoronavirus Infection.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Maria (Verified Customer) (07-06-2022) | Very good antibody, we achieved good results.
|
FH Kyosuke (Verified Customer) (06-12-2019) | it works good for WB.
|
