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HSP90AB1 Polyclonal antibody
HSP90AB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 11405-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue, rat thymus tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 29 publications below |
IHC | See 3 publications below |
IF | See 2 publications below |
IP | See 3 publications below |
CoIP | See 2 publications below |
RIP | See 1 publications below |
Product Information
11405-1-AP targets HSP90AB1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, chicken, sheep, deer |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HSP90AB1 fusion protein Ag1947 |
Full Name | heat shock protein 90kDa alpha (cytosolic), class B member 1 |
Calculated Molecular Weight | 723 aa, 83 kDa |
Observed Molecular Weight | 83-90 kDa |
GenBank Accession Number | BC014485 |
Gene Symbol | HSP90AB1 |
Gene ID (NCBI) | 3326 |
RRID | AB_2121207 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
IHC protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
IF protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
IP protocol for HSP90AB1 antibody 11405-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. | ||
Cell Rep CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons | ||
Haematologica Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. | ||
Anal Chem Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale. | ||
PLoS Pathog A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Maria (Verified Customer) (07-06-2022) | Very good antibody, we achieved good results.
|
FH Kyosuke (Verified Customer) (06-12-2019) | it works good for WB.
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