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HSN2 Polyclonal antibody

HSN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24055-1-AP

Synonyms

HSAN2, HSN2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using HSN2 Polyclonal antibody (24055-1-AP)

    WB analysis of mouse brain using 24055-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24055-1-AP (HSN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HSN2 Polyclonal antibody (24055-1-AP)

    WB analysis of MCF-7 using 24055-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 24055-1-AP (HSN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HSN2 Polyclonal antibody (24055-1-AP)

    IHC staining of human kidney using 24055-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HSN2 Polyclonal antibody (24055-1-AP)

    IHC staining of human kidney using 24055-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using HSN2 Polyclonal antibody (24055-1-AP)

    IHC staining of human gliomas using 24055-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using HSN2 Polyclonal antibody (24055-1-AP)

    IHC staining of human gliomas using 24055-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24055-1-AP (HSN2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells
Positive IHC detected inhuman kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24055-1-AP is HSN2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSN2 fusion protein Ag21184
Full Name hereditary sensory neuropathy, type II
Calculated Molecular Weight 2634 aa, 280 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC130469
Gene Symbol HSN2
Gene ID (NCBI) 378465
RRIDAB_2879417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSN2, also named as WNK1, KDP, KIAA0344, PRKWNK1, P65 and hWNK1, is a serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. It acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. WNK1 may also play a role in actin cytoskeletal reorganization. This antibody detects the 65 kDa protein (Erythrocyte 65 kDa protein, P65).

Protocols

Product Specific Protocols
WB protocol for HSN2 antibody 24055-1-APDownload protocol
IHC protocol for HSN2 antibody 24055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols