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HSD17B8 Polyclonal antibody

HSD17B8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16752-1-AP

Synonyms

17 beta HSD 8, D6S2245E, FABG, FABGL, H2 KE6, HKE6, HSD17B8, Ke 6, KE6, Protein Ke6, RING2, SDR30C1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B8 Polyclonal antibody (16752-1-AP)

    WB analysis using 16752-1-AP

    Hela cells lysates were subjected to SDS PAGE followed by western blot with 16752-1-AP (HSD17B8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    WB analysis of human testis using 16752-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16752-1-AP (HSD17B8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of mouse kidney using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of mouse kidney using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse small intestine tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16752-1-AP targets HSD17B8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B8 fusion protein Ag10226
Full Name hydroxysteroid (17-beta) dehydrogenase 8
Calculated Molecular Weight 261 aa, 27 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008185
Gene Symbol HSD17B8
Gene ID (NCBI) 7923
RRIDAB_2248540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HSD17B8 antibody 16752-1-APDownload protocol
IHC protocol for HSD17B8 antibody 16752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Cell facilitation promotes growth and survival under drug pressure in breast cancer

Authors - Rena Emond
View Article