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HMGCS1 Polyclonal antibody
HMGCS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17643-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, A549 cells, HepG2 cells, LNCaP cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 36 publications below |
| IHC | See 7 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
17643-1-AP targets HMGCS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HMGCS1 fusion protein Ag11825 |
| Full Name | 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) |
| Calculated Molecular Weight | 520 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC083514 |
| Gene Symbol | HMGCS1 |
| Gene ID (NCBI) | 3157 |
| RRID | AB_2248359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HMGCS1(Hydroxymethylglutaryl-CoA synthase, cytoplasmic) is also named as HMGCS and belongs to the HMG-CoA synthase family. The gene encodes a 520 amino acid protein which catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA into 3-hydroxy-3-methylglutaryl CoA and participates in the mevalonate pathway but its end product is cholesterol(PMID:20346956). The human and hamster enzymes are distinguished by differential inhibition by magnesium ion(PMID:2870496).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IHC protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IF protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| IP protocol for HMGCS1 antibody 17643-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis
| ||
Nat Cancer Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism
| ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
J Cell Biol TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination. | ||
Oxid Med Cell Longev Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation. | ||
Mol Cell Proteomics Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH K (Verified Customer) (12-20-2020) | Used for western blotting (1:1000) and IHC (1:200), worked well for me
|
FH H (Verified Customer) (07-09-2020) | This antibody is truly good. No non-specific band detected. The target band is very strong when 15ug of cell lysate was loaded.
 |
FH Boyan (Verified Customer) (07-08-2019) | This antibody recognises a clean, and clear band at the expected molecular weight. It goes down when you treat the cells with cholesterol.
|
