HLA-DRB1 Polyclonal antibody

HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15862-1-AP

Synonyms

Human leukocyte antigen DRB1, HLA DRB1, HLA DRB, HLA DR1B, HLA class II histocompatibility antigen, DRB1 beta chain



Tested Applications

Positive WB detected inRaji cells, human spleen tissue, A375 cells, Daudi cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15862-1-AP targets HLA-DRB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB1 fusion protein Ag8405
Full Name major histocompatibility complex, class II, DR beta 1
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC007920
Gene Symbol HLA-DRB1
Gene ID (NCBI) 3123
RRIDAB_1639897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IHC protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IF protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IP protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation.

Authors - Semini Geo G
humanWB

Nat Commun

Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes

Authors - Rupert L Mayer
mouseWB

Heliyon

Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation

Authors - Qian Gao
humanIHC

Front Pharmacol

Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis

Authors - Yanling Wang