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HDDC3 Polyclonal antibody

HDDC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21091-1-AP

Synonyms

HD domain containing 3, HD domain containing protein 3, HDDC3, MESH1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis using 21091-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis of mouse testis using 21091-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis of mouse kidney using 21091-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis of mouse heart using 21091-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis of HeLa using 21091-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis of human heart using 21091-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis of mouse lung using 21091-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    WB analysis of mouse pancreas using 21091-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HDDC3 Polyclonal antibody (21091-1-AP)

    IP experiment of HeLa using 21091-1-AP

    IP result of anti-HDDC3 (IP:21091-1-AP, 4ug; Detection:21091-1-AP 1:500) with HeLa cells lysate 3400ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human prostate cancer using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human prostate cancer using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human breast cancer using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human kidney using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human kidney using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human breast cancer using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human brain using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HDDC3 Polyclonal antibody (21091-1-AP)

    IHC staining of human brain using 21091-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, human heart tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue, mouse pancreas tissue, mouse testis tissue, A549 cells, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21091-1-AP is HDDC3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDDC3 fusion protein Ag15342
Full Name HD domain containing 3
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC033794
Gene Symbol HDDC3
Gene ID (NCBI) 374659
RRIDAB_10733121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MESH1, encodes by HDDC3 in human and Q9VAM9 in Drosophila melanogaster, was identified as functional SpoT orthologs in metazoan (PMID: 20818390). MESH1 is a cytosolic NADPH phosphatase that is induced under stress conditions, leading to the NADPH depletion and ferroptosis-a novel form of iron-dependent regulated cell death characterized by lipid peroxidation. Accordingly, MESH1 removal preserves the NADPH level in stressed cells and promotes their ferroptotic survival(PMID: 32462112, PMID: 34294679).

Protocols

Product Specific Protocols
WB protocol for HDDC3 antibody 21091-1-APDownload protocol
IHC protocol for HDDC3 antibody 21091-1-APDownload protocol
IP protocol for HDDC3 antibody 21091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

MESH1 knockdown triggers proliferation arrest through TAZ repression.

Authors - Tianai Sun
View Article