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HDAC9 Polyclonal antibody

HDAC9 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23328-1-AP

Synonyms

HD9, EC:3.5.1.98, Histone deacetylase 9, HDRP, HDAC7B

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human testis tissue using HDAC9 Polyclonal antibody (23328-1-AP)

    IHC staining of human testis using 23328-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23328-1-AP (HDAC9 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HDAC9 Polyclonal antibody (23328-1-AP)

    IHC staining of human testis using 23328-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23328-1-AP (HDAC9 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using HDAC9 Polyclonal antibody (23328-1-AP)

    IF Staining of SH-SY5Y using 23328-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC9 antibody (23328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23328-1-AP targets HDAC9 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC9 fusion protein Ag19930
Full Name histone deacetylase 9
Calculated Molecular Weight 1011 aa, 111 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC152405
Gene Symbol HDAC9
Gene ID (NCBI) 9734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HDAC9 antibody 23328-1-APDownload protocol
IF protocol for HDAC9 antibody 23328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols