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KD/KO Validated

HDAC4 Polyclonal antibody

Name: HDAC4 Polyclonal antibody
Brand: Proteintech
SKU: 17449-1-AP

HDAC4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, IP, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17449-1-AP

Synonyms

HA6116, HD4, HDAC A, HDAC4, HDACA, histone deacetylase 4, KIAA0288

Validation Data Gallery

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AllWBIPIHC

WB analysis using 17449-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17449-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17449-1-AP (HDAC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17449-1-AP
IP result of anti-HDAC4 (IP:17449-1-AP, 4ug; Detection:17449-1-AP 1:500) with HeLa cells lysate 4000ug.
IHC staining of human kidney using 17449-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human cervical cancer using 17449-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17449-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17449-1-AP (HDAC4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 17449-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17449-1-AP (HDAC4 Antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 17449-1-AP

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Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, HEK-293T cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 26 publications below
IHC	See 7 publications below
IF	See 6 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below

Product Information

17449-1-AP targets HDAC4 in WB, IHC, IF-P, IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HDAC4 fusion protein Ag11572
Full Name	histone deacetylase 4
Calculated Molecular Weight	972 aa, 106 kDa
Observed Molecular Weight	119-140 kDa
GenBank Accession Number	BC039904
Gene Symbol	HDAC4
Gene ID (NCBI)	9759
RRID	AB_2118864
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against an internal region of human HDAC4. The calculated molecular weight of HDAC4 is 106 kDa, but modified HDAC4 is about 140 kDa.

Protocols

Product Specific Protocols
WB protocol for HDAC4 antibody 17449-1-AP	Download protocol
IHC protocol for HDAC4 antibody 17449-1-AP	Download protocol
IP protocol for HDAC4 antibody 17449-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Cell MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation. Authors - Xiaorong Zhang KD Validated View Article
human	WB, IF	Hepatology Histone Deacetylase 4 promotes cholestatic liver injury in the absence of Prohibitin-1. Authors - Lucía Barbier-Torres KD Validated View Article
	WB,IHC,IF	Kidney Int Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Authors - Xiaojie Wang KD Validated View Article
human	ChIP,CoIP	Mol Ther Nucleic Acids Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity. Authors - Qianqian Guo View Article
human	WB	J Cell Mol Med Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming. Authors - Shoujie Feng KD Validated View Article
human	WB	J Am Heart Assoc Phosphatidylinositol 3-Kinase-DNA Methyltransferase 1-miR-1281-Histone Deacetylase 4 Regulatory Axis Mediates Platelet-Derived Growth Factor-Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells. Authors - Yanjiao Li View Article

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HDAC4 Polyclonal antibody

HDAC4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 17449-1-AP

WB analysis of HEK-293 using 17449-1-AP

IP experiment of HeLa using 17449-1-AP

IHC staining of human kidney using 17449-1-AP

IHC staining of human cervical cancer using 17449-1-AP

IHC staining of human kidney using 17449-1-AP

IHC staining of human cervical cancer using 17449-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Histone Deacetylase 4 promotes cholestatic liver injury in the absence of Prohibitin-1.

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity.

Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Phosphatidylinositol 3-Kinase-DNA Methyltransferase 1-miR-1281-Histone Deacetylase 4 Regulatory Axis Mediates Platelet-Derived Growth Factor-Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells.