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  • Featured Product
  • KD/KO Validated

Histone H2A.z Polyclonal antibody

Histone H2A.z Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16441-1-AP

Synonyms

H2AFZ, Histone H2A, H2AZ1, H2AZ, H2A/z

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Histone H2A.z Polyclonal antibody (16441-1-AP)

    WB analysis of human brain using 16441-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Histone H2A.z Polyclonal antibody (16441-1-AP)

    WB analysis of mouse kidney using 16441-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (Histone H2A.z Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Histone H2A.z Polyclonal antibody (16441-1-AP)

    WB analysis of HepG2 using 16441-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Histone H2A.z Polyclonal antibody (16441-1-AP)

    IHC staining of human skin cancer using 16441-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using Histone H2A.z Polyclonal antibody (16441-1-AP)

    IHC staining of human testis using 16441-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H2A.z Polyclonal antibody (16441-1-AP)

    IF Staining of HeLa using 16441-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, mouse kidney tissue
Positive IHC detected inhuman skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16441-1-AP targets Histone H2A.z in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, fish, oratosquilla oratoria
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2A.z fusion protein Ag9762
Full Name H2A histone family, member Z
Calculated Molecular Weight 128 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC018002
Gene Symbol Histone H2A.z
Gene ID (NCBI) 3015
RRIDAB_2115113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Histone H2A.z antibody 16441-1-APDownload protocol
IHC protocol for Histone H2A.z antibody 16441-1-APDownload protocol
IF protocol for Histone H2A.z antibody 16441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
fishIF

Sci Adv

The lungfish cocoon is a living tissue with antimicrobial functions.

Authors - Ryan Darby Heimroth
View Article
mouseWB

J Biol Chem

The chromatin remodeler protein Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes.

Authors - Haixin Zhao
  • KD Validated
View Article
humanWB

Biomed Pharmacother

Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.

Authors - Ling Gao
View Article
mouseIF,WB

Biol Open

Loss of Elp1 perturbs histone H2A.Z and the Notch signaling pathway.

Authors - BreAnna Cameron
View Article
Oratosquilla oratoriaIF

J Morphol

Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda).

Authors - Tingrong Chen
View Article
humanWB

Front Oncol

DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway

Authors - Zhitao Han
View Article