Filter: AllIHC

IHC staining of human brain using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26950-1-AP (GnRH antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26950-1-AP (GnRH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26950-1-AP (GNRH1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26950-1-AP (GNRH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26950-1-AP (GNRH1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26950-1-AP (GNRH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26950-1-AP (GnRH antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human testis using 26950-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26950-1-AP (GnRH antibody) at dilution of 1:400 (under 40x lens).

Featured Product
KD/KO Validated

GNRH1 Polyclonal antibody

Name: GNRH1 Polyclonal antibody
Brand: Proteintech
SKU: 26950-1-AP

GNRH1 Polyclonal Antibody for IHC, ELISA

Cat No. 26950-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

GNRH, GnRH associated peptide I, GnRH I, GNRH1, Gonadoliberin I, Gonadorelin, GRH, LHRH, LNRH, Luliberin I, Progonadoliberin 1, Progonadoliberin I

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in

human brain tissue, mouse brain tissue, rat brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

26950-1-AP targets GNRH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GNRH1 fusion protein Ag25667 Predict reactive species
Full Name	gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)
Calculated Molecular Weight	92 aa, 10 kDa
GenBank Accession Number	BC126463
Gene Symbol	GNRH1
Gene ID (NCBI)	2796
RRID	AB_2880698
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01148
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for GNRH1 antibody 26950-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Endocrinol (Lausanne) The Anti-proliferative Activity of GnRH Through Downregulation of the Akt/ERK Pathways in Pancreatic Cancer. Authors - Linna Suo KD Validated View Article
pig	IHC,WB	Toxins (Basel) The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts. Authors - Boyang Wan View Article
mouse	IF	bioRxiv Expression of the ACE2 virus entry protein in the nervus terminalis reveals the potential for an alternative route to brain infection in COVID-19. Authors - Katarzyna Bilinska View Article
human,mouse	IHC,IF	Nat Neurosci A GnRH neuronal population in the olfactory bulb translates socially relevant odors into reproductive behavior in male mice Authors - Laurine Decoster View Article