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Glycophorin A Monoclonal antibody

Glycophorin A Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B12G10

Cat no : 66778-1-Ig

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of human blood using Glycophorin A Monoclonal antibody (66778-1-Ig)

    WB analysis of human blood using 66778-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IHC staining of human placenta using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IHC staining of human placenta using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IHC staining of human spleen using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IHC staining of human spleen using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IHC staining of human stomach cancer using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IHC staining of human stomach cancer using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IF Staining of human placenta using 66778-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Glycophorin A Monoclonal antibody (66778-1-Ig)

    IF Staining of human placenta using 66778-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman placenta tissue, human stomach cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66778-1-Ig targets Glycophorin A in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glycophorin A fusion protein Ag8635
Full Name glycophorin A (MNS blood group)
Calculated Molecular Weight 150 aa, 16 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC005319
Gene Symbol Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_2882124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
WB protocol for Glycophorin A antibody 66778-1-IgDownload protocol
IHC protocol for Glycophorin A antibody 66778-1-IgDownload protocol
IF protocol for Glycophorin A antibody 66778-1-IgDownload protocol
FC protocol for Glycophorin A antibody 66778-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biochem

C-terminally truncation is a prominent post-translational modification of human erythrocyte α-synuclein

Authors - Ryosuke Amagai
View Article