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GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24198-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis of mouse brain using 24198-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis using 24198-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis of HeLa using 24198-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis of mouse brain using 24198-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GSK3B Polyclonal antibody (24198-1-AP)

    IP experiment of mouse brain using 24198-1-AP

    IP result of anti-GSK3B (IP:24198-1-AP, 4ug; Detection:24198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using GSK3B Polyclonal antibody (24198-1-AP)

    IHC staining of human testis using 24198-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GSK3B Polyclonal antibody (24198-1-AP)

    IHC staining of human testis using 24198-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSK3B Polyclonal antibody (24198-1-AP)

    IF Staining of HeLa using 24198-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 24198-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inEthacrynic acid treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

24198-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSK3B fusion protein Ag21438
Full Name glycogen synthase kinase 3 beta
Calculated Molecular Weight 433 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC000251
Gene Symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_2879451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 24198-1-APDownload protocol
IHC protocol for GSK3B antibody 24198-1-APDownload protocol
IF protocol for GSK3B antibody 24198-1-APDownload protocol
IP protocol for GSK3B antibody 24198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cardiovasc Med

Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.

Authors - Dong Ma
View Article
humanWB

Tumour Biol

Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts.

Authors - Yumei Cai
View Article
humanWB

Medicine (Baltimore)

Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts.

Authors - Yumei Cai
View Article

Biomed Res Int

Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling.

Authors - Xueyun Chen
View Article