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GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24198-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis of mouse brain using 24198-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis using 24198-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis of HeLa using 24198-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GSK3B Polyclonal antibody (24198-1-AP)

    WB analysis of mouse brain using 24198-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GSK3B Polyclonal antibody (24198-1-AP)

    IP experiment of mouse brain using 24198-1-AP

    IP result of anti-GSK3B (IP:24198-1-AP, 4ug; Detection:24198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using GSK3B Polyclonal antibody (24198-1-AP)

    IHC staining of human testis using 24198-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GSK3B Polyclonal antibody (24198-1-AP)

    IHC staining of human testis using 24198-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSK3B Polyclonal antibody (24198-1-AP)

    IF Staining of HeLa using 24198-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 24198-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inEthacrynic acid treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

24198-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSK3B fusion protein Ag21438
Full Name glycogen synthase kinase 3 beta
Calculated Molecular Weight 433 aa, 48 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC000251
Gene Symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_2879451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 24198-1-APDownload protocol
IHC protocol for GSK3B antibody 24198-1-APDownload protocol
IF protocol for GSK3B antibody 24198-1-APDownload protocol
IP protocol for GSK3B antibody 24198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genes Dis

Naked cuticle homolog 2 controls the differentiation of osteoblasts and osteoclasts and ameliorates bone loss in ovariectomized mice

Authors - Liying Shan
View Article
humanWB

Front Cardiovasc Med

Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.

Authors - Dong Ma
View Article
humanWB

Tumour Biol

Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts.

Authors - Yumei Cai
View Article
humanWB

Medicine (Baltimore)

Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts.

Authors - Yumei Cai
View Article

Biomed Res Int

Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling.

Authors - Xueyun Chen
View Article