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  • Featured Product
  • KD/KO Validated

GSDMB Polyclonal antibody

GSDMB Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12885-1-AP

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using GSDMB Polyclonal antibody (12885-1-AP)

    WB analysis of COLO 320 using 12885-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GSDMB Polyclonal antibody (12885-1-AP)

    WB analysis of PC-3 using 12885-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human liver cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human liver cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human colon cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human colon cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human colon cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human colon cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human liver cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSDMB Polyclonal antibody (12885-1-AP)

    IHC staining of human liver cancer using 12885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12885-1-AP targets GSDMB in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSDMB fusion protein Ag3895
Full Name gasdermin B
Calculated Molecular Weight 411 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC025682
Gene Symbol GSDMB
Gene ID (NCBI) 55876
RRIDAB_2114762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSDMB antibody 12885-1-APDownload protocol
IHC protocol for GSDMB antibody 12885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Front Immunol

Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma

Authors - Liangmin Fu
  • KD Validated
View Article
humanWB, IHC

Int J Biol Sci

USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway.

Authors - Haiqing He
View Article
humanIHC

Front Oncol

Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma.

Authors - Jia You
View Article
humanWB

Toxicol Appl Pharmacol

Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity

Authors - Anping Song
View Article
humanIHC,IF

Cell Death Dis

Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence

Authors - Ying Zhang
View Article
humanWB

J Allergy Clin Immunol

A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis.

Authors - Ronald A Panganiban
View Article