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GRIP1 Polyclonal antibody

GRIP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22398-1-AP

Synonyms

GRIP, GRIP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GRIP1 Polyclonal antibody (22398-1-AP)

    WB analysis using 22398-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22398-1-AP (GRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using GRIP1 Polyclonal antibody (22398-1-AP)

    WB analysis of mouse testis using 22398-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22398-1-AP (GRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GRIP1 Polyclonal antibody (22398-1-AP)

    IP experiment of mouse brain using 22398-1-AP

    IP result of anti-GRIP1 (IP:22398-1-AP, 4ug; Detection:22398-1-AP 1:1000) with mouse brain tissue lysate 5200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GRIP1 Polyclonal antibody (22398-1-AP)

    IHC staining of human kidney using 22398-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22398-1-AP (GRIP1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GRIP1 Polyclonal antibody (22398-1-AP)

    IHC staining of human kidney using 22398-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22398-1-AP (GRIP1 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22398-1-AP targets GRIP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRIP1 fusion protein Ag18071
Full Name glutamate receptor interacting protein 1
Calculated Molecular Weight 1128 aa, 122 kDa
Observed Molecular Weight 130-135 kDa
GenBank Accession NumberBC115393
Gene Symbol GRIP1
Gene ID (NCBI) 23426
RRIDAB_2879099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GRIP1 antibody 22398-1-APDownload protocol
IHC protocol for GRIP1 antibody 22398-1-APDownload protocol
IP protocol for GRIP1 antibody 22398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Development

Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells.

Authors - Akira Kinoshita
View Article
mouseWB

Neuropharmacology

Regulation of hippocampal long term depression by Neuroligin 1.

Authors - Rui Dang
View Article
mouseWB

J Nutr Biochem

Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline

Authors - Ping Lu
View Article