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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
22398-1-AP targets GRIP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRIP1 fusion protein Ag18071 Predict reactive species |
| Full Name | glutamate receptor interacting protein 1 |
| Calculated Molecular Weight | 1128 aa, 122 kDa |
| Observed Molecular Weight | 130-135 kDa |
| GenBank Accession Number | BC115393 |
| Gene Symbol | GRIP1 |
| Gene ID (NCBI) | 23426 |
| RRID | AB_2879099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3R0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRIP1 antibody 22398-1-AP | Download protocol |
| IHC protocol for GRIP1 antibody 22398-1-AP | Download protocol |
| IP protocol for GRIP1 antibody 22398-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Development Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells. | ||
J Nutr Biochem Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline |
