Glutamate receptor 2 Polyclonal antibody

Glutamate receptor 2 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11994-1-AP

Synonyms

GluA2, GluR 2, GluR B, GluR K2, GLUR2, GLURB, Glutamate receptor 2, GRIA2, HBGR2



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11994-1-AP targets Glutamate receptor 2 in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glutamate receptor 2 fusion protein Ag2662
Full Name glutamate receptor, ionotropic, AMPA 2
Calculated Molecular Weight 883 aa, 99 kDa
Observed Molecular Weight 94-105 kDa
GenBank Accession NumberBC010574
Gene Symbol GRIA2
Gene ID (NCBI) 2891
RRIDAB_2113725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.

Protocols

Product Specific Protocols
WB protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
IHC protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
IF protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
IP protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
FC protocol for Glutamate receptor 2 antibody 11994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
mouse,humanWB,CoIP

Sci Adv

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission

Authors - Pengcheng Ma
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin
humanWB

J Clin Invest

TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.

Authors - Haiqing Zhang
mouseWB

Mol Neurobiol

A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons.

Authors - Davide Capauto
mouseIP

Cell Death Dis

ZDHHC8 critically regulates seizure susceptibility in epilepsy.

Authors - Qin Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is quite strong (50-70sec of exposure) and gives "clean" bands. The diluted antibody (TBS 0.1% Tween+5% BSA) does not work well anymore after 2-3 times of re-use

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: hiPSCs-derived neurons