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GRB2 Polyclonal antibody
GRB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 10254-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, rat brain tissue, rat spleen tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
10254-2-AP targets GRB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRB2 fusion protein Ag0395 |
| Full Name | growth factor receptor-bound protein 2 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC000631 |
| Gene Symbol | GRB2 |
| Gene ID (NCBI) | 2885 |
| RRID | AB_2263577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRB2 antibody 10254-2-AP | Download protocol |
| IHC protocol for GRB2 antibody 10254-2-AP | Download protocol |
| IF protocol for GRB2 antibody 10254-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway. | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Cancer Lett Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2. | ||
Food Funct Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel | ||
Cancer Lett Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma. |
