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CD42a / GP9 Polyclonal antibody

CD42a / GP9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14564-1-AP

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD42a / GP9 Polyclonal antibody (14564-1-AP)

    WB analysis of human peripheral blood platelets using 14564-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 14564-1-AP (CD42a / GP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD42a / GP9 Polyclonal antibody (14564-1-AP)

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD42a / GP9 Polyclonal antibody (14564-1-AP)

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHuman peripheral blood platelets
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14564-1-AP targets CD42a / GP9 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD42a / GP9 fusion protein Ag6129
Full Name glycoprotein IX (platelet)
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC030229
Gene Symbol CD42a
Gene ID (NCBI) 2815
RRIDAB_2878067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

Protocols

Product Specific Protocols
WB protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
IHC protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Death Dis

Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression

Authors - Rong Yan
View Article