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  • Featured Product
  • KD/KO Validated

GNG4 Polyclonal antibody

GNG4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13780-1-AP

Synonyms

G gamma4, GNG4, GNGT4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNG4 Polyclonal antibody (13780-1-AP)

    WB analysis using 13780-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13780-1-AP (GNG4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GNG4 Polyclonal antibody (13780-1-AP)

    IHC staining of human pancreas cancer using 13780-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13780-1-AP (GNG4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GNG4 Polyclonal antibody (13780-1-AP)

    IHC staining of human pancreas cancer using 13780-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13780-1-AP (GNG4 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inSW480 cells, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13780-1-AP targets GNG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNG4 fusion protein Ag4787
Full Name guanine nucleotide binding protein (G protein), gamma 4
Calculated Molecular Weight 75 aa, 8 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC022485
Gene Symbol GNG4
Gene ID (NCBI) 2786
RRIDAB_2109909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GNG4 antibody 13780-1-APDownload protocol
IHC protocol for GNG4 antibody 13780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Br J Cancer

G-protein subunit gamma-4 expression has potential for detection, prediction and therapeutic targeting in liver metastasis of gastric cancer.

Authors - Haruyoshi Tanaka
  • KD Validated
View Article
mouseWB

Acta Pharm Sin B

Engagement of N6-methyladenisine methylation of Gng4 mRNA in astrocyte dysfunction regulated by CircHECW2

Authors - Ying Bai
View Article