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GNB2 Polyclonal antibody

GNB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16090-1-AP

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GNB2 Polyclonal antibody (16090-1-AP)

    WB analysis of HeLa using 16090-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GNB2 Polyclonal antibody (16090-1-AP)

    WB analysis of HEK-293 using 16090-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GNB2 Polyclonal antibody (16090-1-AP)

    WB analysis of Jurkat using 16090-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GNB2 Polyclonal antibody (16090-1-AP)

    WB analysis of HeLa using 16090-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GNB2 Polyclonal antibody (16090-1-AP)

    IHC staining of human ovary tumor using 16090-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GNB2 Polyclonal antibody (16090-1-AP)

    IHC staining of human ovary tumor using 16090-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GNB2 Polyclonal antibody (16090-1-AP)

    IF Staining of HepG2 using 16090-1-AP

    Immunofluorescent analysis of HepG2 cells, using GNB2 antibody 16090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16090-1-AP targets GNB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB2 fusion protein Ag9125
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 2
Calculated Molecular Weight 340 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC010073
Gene Symbol GNB2
Gene ID (NCBI) 2783
RRIDAB_2111939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNB2(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2) is also named as G protein subunit beta-2, transducin beta chain 2 and belongs to the WD repeat G protein beta family. G proteins are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Protocols

Product Specific Protocols
WB protocol for GNB2 antibody 16090-1-APDownload protocol
IHC protocol for GNB2 antibody 16090-1-APDownload protocol
IF protocol for GNB2 antibody 16090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Front Cardiovasc Med

Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.

Authors - Yue Zheng
View Article