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GNAQ Polyclonal antibody

GNAQ Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27264-1-AP

Synonyms

G ALPHA q, GAQ, GNAQ

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using GNAQ Polyclonal antibody (27264-1-AP)

    WB analysis of PC-12 using 27264-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GNAQ Polyclonal antibody (27264-1-AP)

    WB analysis of mouse brain using 27264-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GNAQ Polyclonal antibody (27264-1-AP)

    WB analysis of HepG2 using 27264-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPC-12 cells, HepG2 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27264-1-AP is GNAQ Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAQ fusion protein Ag26170
Full Name guanine nucleotide binding protein (G protein), q polypeptide
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC057777
Gene Symbol GNAQ
Gene ID (NCBI) 2776
RRIDAB_2880822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Protocols

Product Specific Protocols
WB protocol for GNAQ antibody 27264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols